Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872583 | FEBS Letters | 2007 | 5 Pages |
Abstract
Serotonin release from preloaded pancreatic β-cells has been used as a marker for insulin release in studying exocytosis from single cells using the amperometric technique. We found that single pancreatic β-cells exhibited oscillations in exocytosis with a period of 1-1.5 min as measured amperometrically by serotonin release. We also show that 3H-serotonin can be used to monitor exocytosis from intact and streptolysin-O permeabilized clonal insulin-secreting cells preloaded with labeled serotonin and that serotonin release correlated with insulin secretion in the same cells. The use of 3H-serotonin provides a real-time indicator of exocytosis from populations of clonal insulin-secreting cells.
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Authors
Jude T. Deeney, Robert Bränström, Barbara E. Corkey, Olof Larsson, Per-Olof Berggren,