| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10872607 | FEBS Letters | 2007 | 9 Pages |
Abstract
We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.
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Authors
Ji-Kang Fang, Subbuswamy K. Prabu, Naresh B. Sepuri, Haider Raza, Hindupur K. Anandatheerthavarada, Domenico Galati, Joseph Spear, Narayan G. Avadhani,