Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872618 | FEBS Letters | 2007 | 8 Pages |
Abstract
Here we present the crystal structure of Importin-β(1-462) · Ran · GTP · RanBD1ÎN as solved by molecular replacement. HPLC dissociation measurements on this complex show, that the N-terminus of RanBD may be involved in the release of the hydrolysis- and dissociation-block of Ran by Transportin/Importin-β. We could identify a pair of amino acids which - upon mutation - weaken the interaction between Ran and Importin-β specifically to allow dissociation without RanBD. These findings support the hypothesis that a ternary complex of Importin-β · Ran · GTP · RanBD exists in the final step of the export of Importin-β from the nucleus and that interaction of the N-terminus of RanBD with Ran plays a crucial role in disassembly of this complex.
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Authors
Marc SariÄ, Xiaodong Zhao, Carolin Körner, Christine Nowak, Jürgen Kuhlmann, Ingrid R. Vetter,