Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872631 | FEBS Letters | 2007 | 7 Pages |
Abstract
Non-steroidal anti-inflammatory drugs (NSAIDs), such as indomethacin, have serious gastrointestinal side effects. Since their direct cytotoxicity was suggested to be involved in this side effect, we here tried to identify NSAID-resistant genes. We screened for Saccharomyces cerevisiae genes whose overexpression causes indomethacin resistance and identified the TPO1 gene, which encodes a major facilitator superfamily transporter. Its overexpression or deletion made yeast cells resistant or sensitive, respectively, to some NSAIDs. A BLAST search identified the possible human orthologue of Tpo1p, tetracycline transporter-like protein (TETRAN), whose overexpression in cultured human cells caused resistance to some NSAIDs, suggesting that TETRAN is an efflux pump for some NSAIDs.
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Authors
Shinji Mima, Hironori Ushijima, Hyun-Jung Hwang, Shinji Tsutsumi, Masaki Makise, Yoshihiro Yamaguchi, Tomofusa Tsuchiya, Hiroshi Mizushima, Tohru Mizushima,