Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872731 | FEBS Letters | 2005 | 6 Pages |
Abstract
The influence of the enhancer of split- and hairy-related protein-2 (SHARP-2) transcriptional repressor on the expression of rat phosphoenolpyruvate carboxykinase (PEPCK) gene was examined. When H4IIE cells were treated with epigallocatechin gallate, a green tea constituent, an increase in SHARP-2 mRNA levels and a decrease in PEPCK mRNA levels were observed. The adenovirus-mediated overexpression of SHARP-2 in H4IIE cells and primary cultured rat hepatocytes led to a decrease in the levels of PEPCK mRNA. Finally, when a SHARP-2 expression plasmid was transiently transfected with various reporter plasmids into MH1C1 cells, the promoter activity of a PEPCK reporter plasmid was specifically decreased. Based on these findings, we conclude that SHARP-2 is a potential repressor of PEPCK gene expression.
Keywords
EGCGPI 3-Km.o.i.36B4Stra13PEPCKPKBIRSSREBP-1cDEXDMEM(−)-epigallocatechin gallateinsulinTranscriptional regulationDexamethasonephosphoenolpyruvate carboxykinasephosphoinositide 3-kinaseDulbecco’s modified eagle’s mediumpolymerase chain reactionPCRsterol regulatory element-binding protein-1cprotein kinase Bmultiplicity of infectionGlucokinase
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Authors
Kazuya Yamada, Hiroko Ogata-Kawata, Kaoru Matsuura, Kaoru Miyamoto,