Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872734 | FEBS Letters | 2005 | 5 Pages |
Abstract
Though the role of Hedgehog (Hh) signalling in patterning and differentiation during development is well established, the underlying signal transduction mechanisms remain obscure. This is the first report on the overexpression of the human Hh signalling receptor Smoothened (hSmo) in Saccharomyces cerevisiae and Pichia pastoris. We show that hSmo is expressed in both types of yeast in its native conformational state. The first purification presented here will allow the characterisation of hSmo expressed in yeast, and the scale-up of hSmo production enabling structural studies to develop new therapeutic approaches against tumors and neurodegenerative diseases induced by Hh signalling dysfunction.
Keywords
ethylene glycol bis(β-aminoethyl ether)AOX1DDAOAlcohol oxidase 1hemagglutinin APTCPMSFEGTACHSCBDDDMn-Dodecyl-β-D-maltosideSDS–PAGEsodium dodecyl sulfate–polyacrylamide gel electrophoresisHeterologous expressionHedgehogCalmodulin binding domainHedgehog signallingSmoothenedphenylmethylsulfonyl fluorideYeastpolymerase chain reactionPCRPurificationMembrane proteinPatchedCHAPSCholesteryl hemisuccinate
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Authors
Matthieu De Rivoyre, Frédéric Bonino, Laurent Ruel, Michel Bidet, Pascal Thérond, Isabelle Mus-Veteau,