Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872816 | FEBS Letters | 2005 | 5 Pages |
Abstract
To explore how the sulfonylurea receptor (SUR1) is involved in docking and fusion of insulin granules, dynamic motion of single insulin secretory granules near the plasma membrane was examined in SUR1 knock-out (Sur1KO) β-cells by total internal reflection fluorescence microscopy. Sur1KO β-cells exhibited a marked reduction in the number of fusion events from previously docked granules. However, the number of docked granules declined during stimulation as a consequence of the release of docked granules into the cytoplasm vs. fusion with the plasma membrane. Thus, the impaired docking and fusion results in decreased insulin exocytosis from Sur1KO β-cells.
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Authors
Toshiteru Kikuta, Mica Ohara-Imaizumi, Mitsuhiro Nakazaki, Chiyono Nishiwaki, Yoko Nakamichi, Chuwa Tei, Lydia Aguilar-Bryan, Joseph Bryan, Shinya Nagamatsu,