Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872912 | FEBS Letters | 2005 | 5 Pages |
Abstract
This study demonstrated that activation of tyrosine kinase of epidermal growth factor receptor (EGFR) induces its association with G protein-coupled receptor kinase 2 (GRK2). Immunoprecipitation experiments showed that EGF stimulation increased GRK2 binding to EGFR complex in HEK293 cells coexpressing EGFR and GRK2. The EGF-induced GRK2-EGFR complex formation was greatly reduced by perturbation of EGFR and Src tyrosine kinase activity. Furthermore, studies with GRK2 mutants showed that neither catalytic activity nor the N-terminal domain of GRK2 was required for EGF-induced GRK2-EGFR complex formation. However, overexpression of Gβγ scavengers blocked EGF-induced formation of GRK2-EGFR complex.
Keywords
EGFRGβγGPCR kinasePDGFRGRKPTXGPCRSRCHEKGFPEGFG protein-coupled receptor kinaseSDS–PAGEpolyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfatepertussis toxinepidermal growth factorPhosphorylationhemagglutiningreen fluorescent proteinhuman embryonic kidneyEGF receptorReceptor Tyrosine Kinaseplatelet-derived growth factor receptorEpidermal growth factor receptorG protein-coupled receptor
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Authors
Jingxia Gao, Jiali Li, Yuejun Chen, Lan Ma,