| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10873392 | FEBS Letters | 2005 | 6 Pages |
Abstract
MLL is involved in the process of gene activity maintenance. It is shown that the amino-terminal region of MLL (MLLN) interacts with TAF-Iβ/SET. In this study, using yeast two-hybrid assays, we have found that the acidic region of TAF-Iβ is essential for its binding to MLLN. Pull-down assays using GST-MLLN demonstrated that TAF-Iβ and histones interact with GST-MLLN. MLLN and TAF-Iβ synergistically upregulated the transcription level of Hoxa9 and co-immunoprecipitated in chromatin containing the Hoxa9 promoter region. These results suggest that TAF-Iβ plays an important role in MLL-mediated transcription and possibly chromatin maintenance.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Tae Shimoyama, Kohsuke Kato, Mary Miyaji-Yamaguchi, Kyosuke Nagata,