| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10873609 | FEBS Letters | 2005 | 6 Pages |
Abstract
The influence in vivo of mutated forms of translation initiation factor (IF1) on the expression of the lacZ or 3Aâ² reporter genes, with different initiation and/or +2 codons, has been investigated. Reporter gene expression in these infA(IF1) mutants is similar to the wild-type strain. The results do not support the longstanding hypothesis that IF1 could perform discriminatory functions while blocking the aminoacyl-tRNA acceptor site (A-site) of the ribosome. One cold-sensitive IF1 mutant shows a general overexpression, in particular at low temperatures, of both reporter genes at the protein but not mRNA level.
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Authors
Victor V. Croitoru, Margarete Bucheli-Witschel, Leif A. Isaksson,