Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10873620 | FEBS Letters | 2005 | 6 Pages |
Abstract
Haem controls its own synthesis in non-erythroid cells primarily by regulation of ALAS1 mRNA stability. Alternative splicing of human ALAS1 generates two mRNAs with different 5â²-UTRs: a major one, where exon 1B is omitted, and a minor form containing exon 1B. We show that, unlike the major ALAS1 mRNA, the minor form was resistant to haem-mediated decay. Furthermore, we demonstrate that the ALAS1 5â²-UTR alone did not confer haem-mediated decay upon a heterologous mRNA and the inclusion of exon 1B inhibited translation. These data suggest that translation of ALAS1 mRNA itself might be required for destabilisation in response to haem.
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Authors
Andrew G. Roberts, Sadie J. Redding, David H. Llewellyn,