Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10876918 | Journal of Plant Physiology | 2014 | 4 Pages |
Abstract
Ca2+ is a key player in plant cell responses to biotic and abiotic stress. Owing to the central role of cytosolic Ca2+ ([Ca2+]cyt) during early signaling and the need for precise determination of [Ca2+]cyt variations, we used a Cameleon YC 3.6 reporter protein expressed in Arabidopsis thaliana to quantify [Ca2+]cyt variations upon leaf mechanical damage (MD), herbivory by 3rd and 5th instar larvae of Spodoptera littoralis and S. littoralis oral secretions (OS) applied to MD. YC 3.6 allowed a clear distinction between MD and herbivory and discriminated between the two larvae instars. To our knowledge this is the first report of quantitative [Ca2+]cyt determination upon herbivory using a Cameleon calcium sensor.
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Authors
Francesca Verrillo, Andrea Occhipinti, Chidananda Nagamangala Kanchiswamy, Massimo E. Maffei,