Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10876995 | Journal of Plant Physiology | 2005 | 6 Pages |
Abstract
Successful shoot regeneration of Dendrobium nobile was achieved using thin shoot tip sections and triacontanol (TRIA) for the first time. Protocorm-like bodies (PLBs) or proliferating shoot buds were observed when thin shoot tip sections were cultured on the basal medium of Mitra et al. (Indian J. Exp. Biol. 14 (1976) 350) supplemented with 4.0 μg Lâ1 TRIA. The highest percentage of explants (93%) produced PLBs or proliferating shoot buds (21) at 4.0 μg Lâ1 TRIA-supplemented basal medium. All the newly formed PLBs or proliferating shoot buds survived and ultimately produced healthy shoots with 2-3 leaves. Shoots produced roots when cultured on basal medium supplemented with 2.0 μg Lâ1 TRIA. The well-rooted shoots were transferred to pots containing charcoal chips, coconut husk and broken tiles (2:2:1), and a 92% survival rate was achieved. This work reveals that TRIA can be used as an effective growth regulator in the micropropagation and conservation of D. nobile.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Agronomy and Crop Science
Authors
Ravindra B. Malabadi, Gangadhar S. Mulgund, Nataraja Kallappa,