Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10878240 | Mycoscience | 2005 | 5 Pages |
Abstract
We investigated the characteristics of desaturation in Trichoderma sp. AM076. Although 6,9,12- octadecatrienoic acid (18:3Ï6) was detected when Trichoderma sp. AM076 was cultivated in the presence of 6,9-octadecadienoic acid (18:2w9), the desaturation products of 6,9,12-octadecatrienoic acid (18 :3Ï6) and 6- octadecenoic acid (18:1Î6) were not detected. These results suggest that the double bonds at the Î6 position of 18:3Ï6 and 18 :1Î6 disturb their Î15 and Î9 desaturation, respectively. This fungus also introduced a double bond at the Î15 position of 9,12-hexadecadienoic acid (16:2Ï4), thereby yielding a novel C16 polyunsaturated fatty acid (PUFA) identified as 9,12,15-hexadecatrienoic acid (16:3Ï1). Further investigations revealed that the mutant having enhanced accumulation of linolenic aid (18:3Ï3) accumulates 16:3Ï1 as one of the major PUFAs, together with 9,12-octadecadienoic acid (16 :2Ï4), when grown with palmitoleic acid (16 :1Ï7). These results suggest that, in this strain, the reaction that catalyzes the conversion of linoleic acid to linolenic acid, similar to the conversion of 16 :2Ï4 to 16:3Ï1, is not Ï3 desaturation but Î15 desaturation.
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Authors
Norifumi Shirasaka, Takuya Umehara, Yasunori Fukuda, Hajime Yoshizumi, Sakayu Shimizu,