Ultrastructure of the Algivorous Amoeboflagellate Viridiraptor invadens (Glissomonadida, Cercozoa)

The family Viridiraptoridae represents a morphologically and ecologically distinct lineage of glissomonad flagellates (Cercozoa, Rhizaria). It currently comprises two highly specialised, algivorous genera inhabiting freshwater ecosystems, Orciraptor and Viridiraptor, for which ultrastructural data were lacking. In this study, the ultrastructure of Viridiraptor invadensHess et Melkonian, the sole described species of the viridiraptorid type genus, has been studied by transmission and scanning electron microscopy. In particular the flagellar transitional region and basal apparatus, both reconstructed from serial sections, revealed ultrastructural traits, that agree with the phylogenetic placement of the viridiraptorids within Glissomonadida: The transitional region contains a distal plate/collar complex and the basal apparatus comprises two ventral posterior roots and an anterior root, all known from other glissomonads. However, two additional small microtubular roots, two conspicuous rhizoplasts and probasal bodies present during interphase represent novel characteristics. Furthermore, an acorn/V-shaped filament system was discovered at the proximal end of the flagellar transitional region and used to establish a basal body triplet numbering system for flagellate cells of the Rhizaria. Finally, ultrastructural data on perforated algal cell walls suggest that the previously described reticulocyst of Viridiraptor represents an extrusome-derived, mesh-like coat supporting the invasion/feeding process.