Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10893067 | Theriogenology | 2011 | 5 Pages |
Abstract
The main objective of this study was to investigate if centrifugation through PureSperm® density-gradient can improve the post-thaw semen quality of dog semen. Semen from 5 dogs was collected and cryopreserved following a standard protocol. After thawing, semen samples were selected by centrifugation on PureSperm®. Assessments of sperm motility (assessed by computerized-assisted semen analysis), morphology (Diff-Quick staining) and viability (triple fluorescent stain of Propidium iodine/isothiocyanate-labeled peanut (Arachis hypogaea) agglutinin/Rhodamine 123), were performed on aliquots of fresh semen, unselected samples and selected preparations. Cryopreservation had a significant (P < 0.001) effect on all studied semen parameters. PureSperm® centrifugation yielded sperm suspensions with improved motility and viability (P < 0.001). The washing step significantly reduced (P < 0.001) all of the kinematics parameters evaluated as well as reduced the proportion of viable spermatozoa with intact acrosomes (P < 0.05). We concluded that PureSperm® centrifugation is a successful method for improving the quality of frozen-thawed dog spermatozoa. However, washing after density-gradient centrifugation dramatically reduces the post-thaw semen quality, indicating that the inclusion of such a washing step is unnecessary.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Animal Science and Zoology
Authors
J. Dorado, L. Alcaráz, N. Duarte, J.M. Portero, D. Acha, S. Demyda, A. Muñoz-Serrano, M. Hidalgo,