Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10894510 | Theriogenology | 2012 | 5 Pages |
Abstract
The Putian Black pig, as one of elite cultivars of endemic species in China, has been on the verge of extinction and urgently needs protection. Somatic cell nuclear transfer (SCNT) and noncryoprotected frozen tissue technology have successfully resurrected several mammalian species. Therefore, this study explored the primary feasibility of conserving this breed using a combination of both technologies. Skin tissues obtained from the ears of adult Putian Black boars were frozen without cryoprotectant at â20, â80, or â196 °C and stored for 3 yrs. Primary cell culture, passage and subculture were performed on frozen samples after being rapidly thawed at 39 °C and on fresh pig ear tissues (control). Cloned embryos were reconstructed using fibroblasts (from frozen and fresh tissues) with enucleated oocytes. Live cell lines were obtained from tissues frozen at â80 and at â196 °C and appeared to have normal proliferative activity after passage; furthermore, they directed cloned embryos to develop to the blastocyst stage after nuclear transfer. We concluded that the population of Putian Black pig might be increased in the future by transferring cloned blastocysts into synchronized recipient pigs.
Keywords
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Agricultural and Biological Sciences
Animal Science and Zoology
Authors
Yu-Ling Zhang, Feng-Jun Liu, Yi-Fen Zhuang, Xiu-Ai Wang, Xiao-Wei Zhai, Hong-Xiang Li, Zhi-Yong Hong, Jun-Jie Chen, Ling-Chao Zhong, Wen-Chang Zhang,