Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10927615 | Cryobiology | 2016 | 6 Pages |
Abstract
Several methods are currently available for selection when conducting sperm cryopreservation, however, these methods might cause different degrees of damage on sperm DNA. The aim of the this study is to compare the effects of storage at â80 °C (in ultra-low temperature refrigerator) and at â196 °C (in liquid nitrogen) on sperm DNA damage, thus to provide a reference for choosing the right method according to different aims. We randomly collected 28 semen samples from college students of Chongqing city. The samples stored at â80 °C were neat semen samples and the samples stored at â196 °C were mixed with additional cryoprotectants. Each sample was subjected to two freezing-thawing cycles, and the sperm DNA damage levels of fresh and thawed samples were measured by single cell gel electrophoresis (SCGE) and sperm chromatin structure assay (SCSA). Both SCGE and SCSA assays showed cryopreservation induced significant damage to sperm DNA. However, storage at â196 °C lead to more severe damage to sperm DNA than storage at â80 °C measured by SCSA. Sperm DNA damage increased simultaneously with the higher frequency of freezing-thawing cycles. We concluded that storage of neat semen samples at â80 °C had milder damage to sperm DNA than storage at â196 °C mixed with cryoprotectants. To avoid additional sperm DNA damage, repeated freezing and thawing should be prevented.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Agricultural and Biological Sciences (General)
Authors
Taixiu Liu, Jianfang Gao, Niya Zhou, Min Mo, Xiaogang Wang, Xi Zhang, Huan Yang, Qing Chen, Lin Ao, Jinyi Liu, Zhihong Cui, Jia Cao,