Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10927624 | Cryobiology | 2016 | 22 Pages |
Abstract
The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6Â h incubation when the extender was supplemented by 5Â mM BHT or cysteamine.
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Authors
Selim Alcay, Elif Gokce, M. Berk Toker, N. Tekin Onder, Burcu Ustuner, Ender Uzabacı, Zulfiye Gul, Seda Cavus,