Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10927636 | Cryobiology | 2016 | 6 Pages |
Abstract
Results showed that the freezing media containing the HPβCD-C complex with 1%, 2% and 3% glycerol significantly improve the 3 in vitro parameters of post thawing semen quality (viability, progressive and total mobilities) compared to IF. The best improvement of the parameters was obtained with G1 medium and the longest contact time. The substitution of egg yolk by HPβCD-C complex allows the decrease of protein charge of the medium while favouring the cholesterol supply to membrane spermatozoa offering it a better resistance to osmotic imbalance and a better tolerance to the glycerol toxicity. Our results highlight that the egg yolk of an extender for the freezing of horse semen can be completely substituted by HPβCD-C complex.
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Authors
Didier Blommaert, Thierry Franck, Isabelle Donnay, Jean-Philippe Lejeune, Johann Detilleux, Didier Serteyn,