Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10927787 | Cryobiology | 2015 | 10 Pages |
Abstract
We developed an in house protocol using the combination of Me2SO/HES/SR medium and ultra-slow programmable freezing system which resulted in hESC colonies that remain undifferentiated, maintain their in vitro and in vivo pluripotency properties and genetic stability. This approach may be suitable for cell therapy studies.
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Authors
Maristela Delgado Orellana, Gil Cunha De Santis, Kuruvilla Joseph Abraham, Aparecida Maria Fontes, Danielle Aparecida Rosa Magalhães, Viviane de Cássia Oliveira, Everton de Brito Oliveira Costa, PatrÃcia Vianna Bonini Palma, Dimas Tadeu Covas,