Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10927864 | Cryobiology | 2015 | 7 Pages |
Abstract
This study was designed to evaluate the capacity of vitrified-warmed porcine immature oocytes to mature and to be fertilized using in vitro fertilization or intracytoplasmic sperm injection, and to determine the subsequent embryo development. Immature oocytes were vitrified using ethylene glycol and dimethylsulphoxide as cryoprotectants and the Cryolock method. After warming oocytes were cultured 44Â h for maturation. Oocytes were randomly distributed in three treatment groups and subjected to in vitro fertilization (Experiment 1) or intracytoplasmic sperm injection (Experiment 2) procedures. The results indicate that the embryo development was higher in denuded oocytes co-cultured with granulosa cells (NkO-CC group) fertilized by in vitro fertilization or intracytoplasmic sperm injection compared to cumulus-cell oocyte complexes (COCs group), showing no significant differences with control. Vitrified denuded oocytes matured with a co-culture system NkO-CC group, displayed higher cleavage rate and blastocyst production than vitrified COCs group. Blastocysts were successfully obtained after IVF and ICSI procedures; however, the development to the blastocyst stage was better after IVF. These results show that the vitrification-warming media, the employment of a granulosa cell co-culture system and the Cryolock method during vitrification, increased the nuclear and cytoplasmic maturation of vitrified porcine immature oocytes. Further experiments are required to enhance porcine embryo production after vitrification.
Keywords
PVATCM-199EGFpronucleiPVPCOCsMe2SOIVMCPAsBSAMTTbovine serum albuminEthylene glycolpolar bodyIn vitro maturationimmature oocytesintracytoplasmic sperm injectionembryo developmentthiazolyl bluedimethylsulphoxidecumulus cellsgranulosa cellsVitrificationepidermal growth factorCryoprotectant agentsIn vitro fertilizationIVFZona pellucidaICSIpolyvinyl alcoholpolyvinylpyrrolidonePorcine
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Authors
Fahiel Casillas, Yvonne Ducolomb, Ana E. Lemus, Cristina Cuello, Miguel Betancourt,