| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10928602 | Cryobiology | 2011 | 7 Pages |
Abstract
⺠In ESC culture quality of MEFs should be regularly checked. ⺠Optimal cryopreservation of 30 Gy γ-irradiated MEF is 1 K/min plus 10% DMSO. ⺠Thawed cells should have 85% viability and be free of mycoplasma. ⺠Cells should have 150% metabolic activity and 225% selected marker gene expression.
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Authors
Ulf Diekmann, Ralf Spindler, Willem F. Wolkers, Birgit Glasmacher, Thomas Müller,