| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10971310 | Comparative Immunology, Microbiology and Infectious Diseases | 2014 | 5 Pages |
Abstract
In the present study, a molecular proteomics (MALDI-TOF/MS) approach was used as a tool for identifying flea vectors. We measured the MS spectra from 38 flea specimens of 5 species including Ctenocephalides felis, Ctenocephalides canis, Archaeopsylla erinacei, Xenopsylla cheopis and Stenoponia tripectinata. A blind test performed with 24 specimens from species included in a library spectral database confirmed that MALDI-TOF/MS is an effective tool for discriminating flea species. Although fresh and 70% ethanol-conserved samples subjected to MALDI-TOF/MS in blind tests were correctly classified, only MS spectra of quality from fresh specimens were sufficient for accurate and significant identification. A cluster analysis highlighted that the MALDI Biotyper can be used for studying the phylogeny of fleas.
Keywords
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Authors
Amina Yssouf, Cristina Socolovschi, Hamza Leulmi, Tahar Kernif, Idir Bitam, Gilles Audoly, Lionel Almeras, Didier Raoult, Philippe Parola,