Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10973030 | Journal of Comparative Pathology | 2014 | 9 Pages |
Abstract
The existence of the oestrogen receptor-negative (ORâ)/progesterone receptor-positive (PR+) phenotype in canine mammary carcinomas (CMCs) is not well understood, although this phenotype was reported consistently in previous studies. In the present study, immunohistochemistry (IHC) was performed to categorize CMCs with the ORâ/PR+ phenotype and compare their clinicopathological features with OR+/PR+ tumours. Of a total of 305 CMCs, 36 (11.8%) were categorized as ORâ/PR+ and showed intermediate characteristics between those of OR+/PR+ and ORâ/PRâ cases. OR mRNA levels were measured in formalin-fixed, paraffin wax-embedded samples using a novel branched-chain DNA assay method. Similar to the IHC result, one-way analysis of variance showed that the mean normalized OR mRNA level of ORâ/PR+ tumours (11.4 ± 16.34) was between that of the ORâ/PRâ (mean 4.7 ± 6.35) and OR+/PR+ (mean 15.8 ± 11.95) (P = 0.033) tumours. Only three of the 36 ORâ/PR+ tumours completely lacked OR mRNA expression. The ORâ/PR+ tumours were not categorized as an independent group nor were they included in the other groups on post-hoc analysis. ORâ/PR+ tumours were associated with factors related to poor prognosis compared with OR+/PR+ tumours, but ORâ/PRâ tumours were associated with the worst prognostic indicators. Further studies are required in order to determine the clinical significance of the ORâ/PR+ phenotype.
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Authors
N.-H. Kim, H.-Y. Lim, K.-S. Im, J.-I. Shin, H.-W. Kim, J.-H. Sur,