Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10973482 | Journal of Dairy Science | 2014 | 6 Pages |
Abstract
The objective was to identify and sequence the sua gene (GenBank no. DQ232760; http://www.ncbi.nlm.nih.gov/genbank/) and detect Streptococcus uberis adhesion molecule (SUAM) expression by Western blot using serum from naturally S. uberis-infected cows in strains of S. uberis isolated in milk from cows with mastitis from geographically diverse areas of the world. All strains evaluated yielded a 4.4-kb sua-containing PCR fragment that was subsequently sequenced. Deduced SUAM AA sequences from those S. uberis strains evaluated shared >97% identity. The pepSUAM sequence located at the N terminus of SUAM was >99% identical among strains of S. uberis. Streptococcus uberis adhesion molecule expression was detected in all strains of S. uberis tested. These results suggest that sua is ubiquitous among strains of S. uberis isolated from diverse geographic locations and that SUAM is immunogenic.
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Authors
Ying Yuan, Oudessa Kerro Dego, Xueyan Chen, Eurife Abadin, Shangfeng Chan, Lauren Jory, Steven Kovacevic, Raul A. Almeida, Stephen P. Oliver,