Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10975997 | Journal of Dairy Science | 2014 | 9 Pages |
Abstract
Cronobacter species are opportunistic foodborne pathogens associated with serious infections in preterm neonates and infants. Based on the epidemiological research, infant formula products are considered to be the main source of infections from this organism. Therefore, accurate methods are required for detection of Cronobacter species. In this study, the specific probe and primers for detection of this organism were designed and verified. The probe-magnetic beads were prepared for sequence capture, followed by PCR assay to detect the target gene. This probe-magnetic separation PCR assay could detect as few as 103 cfu/mL of Cronobacter in artificially contaminated infant formulas in less than 4Â h. The combination of magnetic beads and PCR showed the potential for the detection of Cronobacter in infant formulas and may have applications in the dairy industry.
Keywords
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Animal Science and Zoology
Authors
Feng Xu, Peng Li, Xing Ming, Dong Yang, Hengyi Xu, Xiaoli Wu, Nagendra P. Shah, Hua Wei,