Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10977111 | Journal of Dairy Science | 2012 | 8 Pages |
Abstract
Up to 3% of young children develop milk allergy and this may influence the development of immune-mediated diseases in later life. One protein that has been associated with allergic reactions to ruminant milk is αS1-casein (CN). Studies suggest that goat milk with low levels of αS1-CN may reduce allergenicity of milk, but the dose response to αS1-CN has not been confirmed. In this study, we examined the immune response to varying levels of goat αS1-CN in a mouse model of gastrointestinal allergy. BALB/c mice (aged 5 wk) were given intraperitoneal injections with αS1-CN and aluminum as adjuvant at 1 and 3 wk to sensitize mice to the antigen. In wk 5, groups of fasting mice (n = 8/group) were challenged 4 times on alternate days by intragastric gavage with saline or 2, 10, or 20 mg of αS1-CN. Serum levels of specific IgE, IgG1, and IgG2a antibodies and mouse mast cell protease-I were determined. Interleukin-4, IL-10, and IFN-γ responses to 48-h activation with antigen were measured in cultured splenocytes. We determined that mice sensitized with αS1-CN had higher titers of specific IgG1 and IgE antibodies compared with controls; however, groups challenged with differing doses of αS1-CN did not differ. The group challenged with the highest dose of αS1-CN had a 10-fold increase in mouse mast cell protease-I compared with the group challenged with saline. Both IL-4 and IL-10 were produced in a dose-dependent manner by cultured splenocytes incubated with αS1-CN. Overall, αS1-CN stimulated the production of cytokines associated with allergic disease in a dose-dependent manner. Thus, milk with lower levels of αS1-CN should contribute to a lesser antigenic burden.
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Authors
A.J. Hodgkinson, N.A. McDonald, L.J. Kivits, D.R. Hurford, S. Fahey, C. Prosser,