Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11024946 | Postharvest Biology and Technology | 2019 | 9 Pages |
Abstract
Increasing attention to the resistance of plant pathogenic fungi to fungicides and their residues impels the development of more efficient fungicides with novel mechanisms of action. Camptothecin (CPT-1) is a naturally occurring quinoline alkaloid with significant antineoplastic and pesticidal activities. To evaluate the anti-fungicidal activities of CPT-1 and its derivatives against postharvest mango anthracnose disease and their potential as a lead compounds for fungicide development, CPT-1 and its semisynthetic derivatives (CPT-2-15) in vitro and in vivo against Colletotrichum gloeosporioides were tested. Five of the agents, CPT-1, 16a-thiocamptothecin (CPT-2), 7-ethyl-camptothecin (CPT-6), 9-methoxycamptothecin (CPT-11) and 7-benzyl-chloro-camptothecin (CPT-15) at doses of 20âmgâLâ1 produced the effective mycelial growth inhibition of C. gloeosporioides. Among these, CPT-11 exhibited the strongest inhibition, with EC50 and EC90 values of 1.79 and 7.37âmg Lâ1, respectively. At a dose of 100 mg Lâ1, 10 of the tested derivatives inhibited the germination of C. gloeosporioides spores. In addition, CPT-1, â2, â6, â11 and â15 showed different abilities to inhibit appressorium formation. Dipping treatment with CPT-11 at 500âmgâLâ1 exhibited an equivalent efficiency in suppressing postharvest anthracnose in three different cultivated varieties of mango fruit when compared with the commercial fungicide carbendazim at the same concentration, but it was less effective than prochloraz. Scanning and transmission electron microscopy observations revealed that CPT-11 caused alterations in the hyphal morphology and ultrastructures of C. gloeosporioides, including swelling, abnormal branching, and the rupturing and thickening of cell walls. These findings indicated that CPT-11 could be a potential antifungal lead compound for controlling postharvest mango anthracnose disease through a different mode of action than camptothecin.
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Authors
Gang Feng, Xiao-Shuai Zhang, Zheng-Ke Zhang, Huo-Chun Ye, Ying-Qian Liu, Guan-Zhou Yang, Cheng Chen, Min Chen, Chao Yan, Lan-Ying Wang, Jun-Xiang Zhang, Jing Zhang,