Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11029372 | Journal of Plant Physiology | 2018 | 29 Pages |
Abstract
βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development. FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth. The 35S::βIV-Gal plants do not present any phenotypic changes, neither in their morphology nor in their cell walls. In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
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Authors
LucÃa Izquierdo, Ignacio MartÃn, LucÃa Albornos, Josefina Hernández-Nistal, Pablo Hueso, Berta Dopico, Emilia Labrador,