Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells

Article ID	Journal	Published Year	Pages	File Type
11033981	Veterinary Immunology and Immunopathology	2018	10 Pages	PDF

Abstract

We conclude that ETEC, but not E. faecium, was able to stimulate inflammasome components in porcine MoDC. The present experimental conditions revealed no NLRP3 inflammasome-dependent protective effects of E. faecium during a pathogenic ETEC challenge.

Keywords

NLRP3 E. coli BMDC Lactobacillus rhamnosus FMO RT-qPCR ETEC E. faecalis SLA SSC ASC nucleotide oligomerization domain FSC EHEC APC TBP NOD GM-CSF NLR PBS MoDC CFU L. rhamnosus FITC LPS NLRP6 NLRC4 PBMC IgG FCS BSA E. faecium Enterohemorrhagic Escherichia coli enterotoxigenic Escherichia coli Adenosine Triphosphate ATP bovine serum albumin allophycocyanin EDTA ethylenediamine tetra-acetic acid Escherichia coli inflammasomes Enterococcus faecium Enterococcus faecalis interleukin Interleukin-1β Enzyme-linked immunosorbent assay ELISA standard error of the means Pig fetal calf serum Dendritic cell peripheral blood mononuclear cells Dendritic cells Intestinal epithelial cells Monocyte-derived dendritic cells granulocyte-macrophage colony-stimulating factor phycoerythrin fluorescein isothiocyanate Luria-Bertani lipopolysaccharide MHC major histocompatibility complex Phosphate-buffered saline SEM apoptosis-associated speck-like protein colony-forming units real-time quantitative polymerase chain reaction forward scatter side scatter TATA-binding protein nod-like receptor protein 3 Propidium iodide optical density IEC NOD-like receptors