[Ru(bpy)2(bppp)]2 + binds two different forms of the human telomeric G-quadruplex structure

A new complex [Ru(bpy)2(bppp)]2 + (bpy = 2,2′-bipyridine, bppp = 12-bromo-pyrido[2′,3′:5,6]pyrazino[2,3-f][1,10]phenanthroline) has been synthesized and characterized. The interaction of the title complex with two different forms of the human telomeric G-quadruplexes DNA was explored by means of CD spectroscopy, CD melting, fluorescent intercalator displacement (FID) and molecular docking studies. Results indicated that [Ru(bpy)2(bppp)]2 + not only induce formation of antiparallel G-quadruplex structure in the absence of metal cations, but also have the ability to stabilize the G-quadruplexes DNA.

Graphical abstractA new complex [Ru(bpy)2(bppp)]2 + was synthesized and different binding behavior of two different forms of the human telomeric G-quadruplexes was indicated.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A new complex [Ru(bpy)2(bppp)]2+ was synthesized and characterized. ► Two different forms of the G- quadruplexes DNA binding were explored. ► It induces the formation of antiparallel G-quadruplex structure. ► It can stabilize both forms of the G-quadruplexes DNA.