A highly sensitive and selective ratiometric and colorimetric sensor for Hg2+ based on a rhodamine–nitrobenzoxadiazole conjugate

An effective Hg2+-specific probe (1) was designed and prepared based on a rhodamine–nitrobenzoxadiazole conjugate. The sensing behavior of this probe was studied by UV–visible and fluorescence spectroscopy. With Hg2+, the probe displayed both colorimetric responses and ratiometric changes. The ratio of the absorption intensities at 525 and 460 nm for the two chromophores in the probe increased from 0.17 to 0.46. The probe exhibited excellent selectivity and sensitivity towards Hg2+ over other metal ions, which could be attributed to the Hg2+-triggered ring opening of the spirolactam of the rhodamine moiety and the formation of a 1:1 1-Hg2+ complex.

Graphical abstractIntroduction of NBD fluorophore into rhodamine 6G contributes the “naked eye” detection for mercury and builds up a more precise method for monitoring Hg2+ by ratiometric measurement.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Monitoring the qualitative trends by ratiometric measurement. ► Detectable by using “naked eye” and fluorescence methods. ► It is easy to synthesize the probe 1.