| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1305200 | Inorganic Chemistry Communications | 2016 | 9 Pages |
•Three new Ru(II) complexes [Ru(N-N)2(TCPI)](PF6)2 were synthesized and characterized.•The cytotoxicity of the complexes against seven cell lines was evaluated.•The apoptosis, ROS and mitochondrial membrane potential were studied.•The cell invasion was studied under fluorescent microscope.•The cell cycle arrest and Western blot analysis were investigated.
A new ligand TCPI and its three ruthenium(II) polypyridyl complexes [Ru(N-N)2(TCPI)](PF6)2 (N-N = bpy: 2,2′-bipyridine 1; phen: phenanthroline 2; dmp: 2,9-dimethyl-1,10-phenanthroline 3) were synthesized and characterized by elemental analysis, ESI-MS, 1H NMR, IR, absorption and emission spectra. The cytotoxic activity in vitro of the ligand and complexes against cancer cells SGC-7901, PC-12, HepG-2, SiHa, Eca-109, HeLa and normal cell LO2 was evaluated by MTT method. Complex 3 shows the highest cytotoxic activity toward SGC-7901 cell among the complexes. Interestingly, the complexes show low or no cytotoxic activity against normal cell LO2. The apoptosis in SGC-7901 cell was investigated with AO/EB staining method. The ROS levels and the changes of mitochondrial membrane potential were studied under fluorescent microscope and flow cytometry. The cell invasion, cell cycle arrest and the expression of Bcl-2 family proteins were studied in detail. The results demonstrate that the complexes induce apoptosis in SGC-7901 cell through a ROS-mediated mitochondrial dysfunction pathway, which was accompanied by the regulation of Bcl-2 family proteins.
Graphical abstractThree new ruthenium(II) polypyridyl complexes were synthesized and characterized. The anticancer activity was investigated by cytotoxicity in vitro, apoptosis, ROS, mitochondrial membrane potential, cell cycle arrest, cell invasion and Western blot analysis.Figure optionsDownload full-size imageDownload as PowerPoint slide
