Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1358736 | Bioorganic & Medicinal Chemistry Letters | 2015 | 4 Pages |
Abstract
l-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugars d-psicose, d-sorbose, l-tagatose and l-fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Zijie Li, Xiaoru Wu, Li Cai, Shenglin Duan, Jia Liu, Peng Yuan, Hideki Nakanishi, Xiao-Dong Gao,