| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1358911 | Bioorganic & Medicinal Chemistry Letters | 2015 | 4 Pages |
Thrombin binding G-quadruplex oligonucleotide containing two TT-dimer fragments and a gold attachment (ODN G1-G) was designed and synthesized with the aim of understanding the TT-dimer effect in G-quadruplex formation. Our results showed that TT-dimer mutation induced by UV light inhibits the formation of and even destroys the G-quadruplex structure, as confirmed by UV, CD and melting temperature measurements. The structural change resulting from TT-dimer formation with DNA was additionally probed and was found to be accompanied by significant gold aggregation that was observed in the form of a signal change from red to blue.
Graphical abstractThe structural change of thrombin binding G-quadruplex resulting from TT-dimer formation was accompanied by significant gold aggregation that was observed as color change from red to blue.Figure optionsDownload full-size imageDownload as PowerPoint slide
