Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1361487 | Bioorganic & Medicinal Chemistry Letters | 2012 | 4 Pages |
Abstract
We report the mechanistic studies of a FAD:NADH reductase (PrnF) involved in arylamine oxygenation. PrnF catalyzes the reduction of FAD via a sequential ordered bi-bi mechanism with NADH as the first substrate to bind and FADH2 as the first product to be released. The residues Asp145 and His146 are proposed as catalytic acid/base residues for PrnF based on pH profile and molecular dynamics simulation studies. These studies provide the first detailed account of the mechanism of the flavin reductase involved in arylamine oxygenation.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Manish Kumar Tiwari, Raushan Kumar Singh, Jung-Kul Lee, Huimin Zhao,