Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1361729 | Bioorganic & Medicinal Chemistry Letters | 2011 | 4 Pages |
Abstract
3-(3-Phenoxybenzyl)amino-β-carboline 2h showed extremely-high activity; the IC50 value was 0.074 μM. To verify 2h-induced cell death types, we observed the chromatin condensation, the DNA fragmentation and activated caspase-3 using Hoechst 33342, agarose electrophoresis and western blot, and suggesting 2h-induced cell death type was apoptosis. Flow cytometry showed that 2h-treated cell was induced SubG1 cell population after G2/M cell cycle arrest. In addition, using affinity chromatography and peptide mass fingerprinting, we found that interacting protein with this compound was α-tubulin protein.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Reiko Ikeda, Masaki Kurosawa, Takazumi Okabayashi, Ayako Takei, Masamichi Yoshiwara, Tadashi Kumakura, Norio Sakai, Osamu Funatsu, Akinori Morita, Masahiko Ikekita, Yumi Nakaike, Takeo Konakahara,