Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1362176 | Bioorganic & Medicinal Chemistry Letters | 2011 | 4 Pages |
Abstract
The synthesis and biophysical properties of R-6′-Me-α-l-LNA, which has a methyl group in the (R) configuration on the 2′,4′-bridging substituent of α-l-LNA, is reported. The synthesis of the uracil nucleobase phosphoramidite was efficiently accomplished in 14 steps and 8 chromatographic purifications starting from a known sugar intermediate. Biophysical evaluation revealed that substitution along the edge of the major groove does not impair the high affinity duplex forming ability of α-l-LNA modified oligonucleotides.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Punit P. Seth, Jinghua Yu, Charles R. Allerson, Andres Berdeja, Eric E. Swayze,