| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1362755 | Bioorganic & Medicinal Chemistry Letters | 2010 | 5 Pages |
Abstract
An autonomous DNA machine recycling the output as the input for isothermal, sensitive, and specific detection of miRNAs has been developed. This machine shows considerably high signal amplification efficiency (∼1000-fold) and thus a low detection limit (∼20 amol). The machine also shows high specificity, discriminating 50 amol of synthetic miRNA from 100-fold larger amounts of its family member and from 100 ng of unrelated total RNAs. Moreover, it is available for practically detecting natural miRNAs in total RNAs.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Atsushi Ogawa,