| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1363015 | Bioorganic & Medicinal Chemistry Letters | 2010 | 4 Pages |
Abstract
A general strategy to enzymatically label acyl carrier proteins (ACPs) of polyketide synthases has been developed. Incorporation of a chloromethyl ketone or vinyl ketone moiety into polyketide chain elongation intermediate mimics allows for the synthesis of CoA adducts. These CoA adducts undergo enzymatic reaction with Sfp, a phosphopantetheinyl transferase, to afford labeled CurB carrier proteins.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Erick K. Leggans, David L. Akey, Janet L. Smith, Robert A. Fecik,