Iodine scanning of a phenazine inhibitor of vacuolar sorting

Affinity reagents are often used to address the target identification problem in chemical genetics. The design of such reagents so that the linker does not occlude interactions with protein targets is an ongoing challenge. This work describes a systematic approach to synthesize derivatives of a bioactive that should avoid interference with binding to targets and be readily converted to affinity reagents.

Graphical abstractThe synthesis and biological activity of N-phenylphenazin-2-amine and 4 iodinated derivatives is reported.Figure optionsDownload full-size imageDownload as PowerPoint slide