Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1364152 | Bioorganic & Medicinal Chemistry Letters | 2009 | 6 Pages |
Abstract
This paper documents a serious problem met during the testing of Gi protein-activating properties of a new series of synthetic compounds by measuring the induced binding of [35S]GTPγS to different subtypes of Gi protein. The problem arose from the strong affinity between [35S]GTPγS and the tested compounds, that are characterized by several (2–4) positive charges and high lipophilicity. Apparently, such affinity yields insoluble, labelled complexes that, also in the absence of Gi protein, are retained on the filters and give rise to false positive results.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Dina Manetti, Lorenzo Di Cesare Mannelli, Silvia Dei, Luca Guandalini, Elisabetta Martini, Martina Banchelli, Carla Ghelardini,