Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1364584 | Bioorganic & Medicinal Chemistry Letters | 2008 | 6 Pages |
DNA-encoded chemical libraries are increasingly being employed for the identification of binding molecules to protein targets of pharmaceutical relevance. Here, we describe the synthesis and characterization of a DNA-encoded chemical library, consisting of 4000 compounds generated by Diels-Alder cycloaddition reactions. The compounds were encoded with unique DNA fragments which were generated through a stepwise assembly process and serve as amplifiable bar codes for the identification and relative quantification of library members.
Graphical abstractSynthesis and characterization of a novel 4000 compound DNA-encoded chemical library based on the Diels-Alder cycloaddition reaction.Figure optionsDownload full-size imageDownload as PowerPoint slide