| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1364586 | Bioorganic & Medicinal Chemistry Letters | 2008 | 4 Pages |
Abstract
Phage display of protein and peptide libraries offers a powerful technology for the selection and isolation of ligands and receptors. To date, the technique has been considered limited to soluble, non-membrane proteins. We report two examples of phage display of full-length, folded and functional membrane proteins. Consistent display required the recently reported KO7+ helper phage. The two proteins, full-length caveolin-1 and HIV gp41, display well on the surface of the phage, and maintain their binding activities as shown by in vitro assays.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Sudipta Majumdar, Agnes Hajduczki, Aaron S. Mendez, Gregory A. Weiss,