Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1364598 | Bioorganic & Medicinal Chemistry Letters | 2008 | 5 Pages |
Abstract
Chemical probes appended with reactive electrophiles afford powerful tools for profiling discrete protein families in living cells. Herein, we have synthesized cell-permeable chemical probes that target fatty acid-associated proteins. These fatty acid-based chemical probes contain acyloxymethylketone or fluorophosphonate functional groups and an alkyne click chemistry tag for visualization of covalently modified proteins by in-gel fluorescence scanning. Our fatty acid-based chemical probe affords new tools to evaluate the activity/expression of lipid-associated proteins that should facilitate their functional characterization and inhibitor discovery.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Anuradha Raghavan, Guillaume Charron, James Flexner, Howard C. Hang,