Simultaneous identification of multiple receptors of natural product using an optimized cDNA phage display cloning

Simultaneously isolating more than one receptor of natural product remains a challenge to chemical genetics. Using cyclosporine A as an affinity probe and an optimized phage display cloning procedure, not only cyclophilin A, but also cyclophilin B was isolated as the full-length gene clone from a human brain cDNA library. This optimized protocol can be used to select protein targets of chemicals dependent on the binding affinity rather than on the relative abundance in cells.

Graphical abstractAn optimized cDNA phage display cloning procedure is reported to simultaneously isolate two protein targets of cyclosporine A dependent on the binding affinity rather than the relative abundance in cells.Figure optionsDownload full-size imageDownload as PowerPoint slide