Molecular recognition of a DNA:RNA hybrid: Sub-nanomolar binding by a neomycin–methidium conjugate

A novel neomycin–methidium conjugate was synthesized. The covalent linkage of the aminoglycoside to an intercalator, a derivative of ethidium bromide, results in a new conjugate capable of selective recognition of the DNA:RNA hybrid duplex. Spectroscopic methods: UV, CD, fluorescence, and calorimetric techniques: DSC and ITC were used to characterize the sub-nanomolar binding displayed by the conjugate for the DNA:RNA hybrid duplex, poly(dA):poly(rU).

Graphical abstractThe synthesis and binding studies of a neomycin conjugate capable of binding DNA:RNA hybrids in the sub-nanomolar range is presented.Figure optionsDownload full-size imageDownload as PowerPoint slide