Preparative purification of polyethylene glycol derivatives with polystyrene-divinylbenzene beads as chromatographic packing

A clear and powerful chromatographic approach to purify polyethylene glycol derivatives at a preparative scale was reported, which was based on the polystyrene-divinylbenzene beads with ethanol/water as eluants. The validity of this method was verified with the reaction mixture of mPEG-Glu and mPEG propionaldehyde diethylacetal (ALD-PEG) as the model. The target products were one-step achieved with the purity of >99% on the polymer resins column at gram scale. The method developed was free from such disadvantages as utility of toxic solvent and narrow application scope, which was combined with conventional approaches. The method developed provided an appealing and attractive alternative methods for purification of PEG derivatives at a preparative scale.

Graphical abstractA clear and powerful purification approach of PEG derivatives was developed on polystyrene-divinylbenzene beads with ethanol/water as eluants and gave the highly pure target products. The novel methods shared the advantages of clearness, reliability and high-performance. The studies suggested that polystyrene-divinylbenzene beads were potent to serve as medium for the purification of PEG derivatives on a preparative scale.Figure optionsDownload full-size imageDownload as PowerPoint slide