Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1366567 | Bioorganic & Medicinal Chemistry Letters | 2007 | 5 Pages |
We constructed a label-free and detector-free aptazyme-based riboswitch sensor for detecting the cofactor of the aptazyme. This riboswitch, which usually suppresses the gene expression with its anti-RBS sequence bound to the RBS of its own mRNA (OFF), activates the translation only when a cofactor is added to release the anti-RBS sequence from itself as a result of cofactor-induced self-cleavage by the aptazyme (ON). The rationally optimized one with β-galactosidase as a reporter gene enabled us to detect the cofactor of the aptazyme visibly with high ON/OFF efficiency.
Graphical abstractWe constructed a label-free and detector-free aptazyme-based riboswitch sensor for detecting the cofactor of the aptazyme.Figure optionsDownload full-size imageDownload as PowerPoint slide